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FAM189B

ANTIBODY INFORMATION
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006659   HPA050674  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA006659, RRID:AB_1078339   Atlas Antibodies Cat#HPA050674, RRID:AB_2681213  

VALIDATION SUMMARY
 
 

Antibody HPA006659

  

Antibody HPA050674

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.  

Antibody dilution

 1:39   1:31  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 61 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:25    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71.4, 69.4, 61.1, 34.9   71.4, 69.4, 61.1  

Antibody dilution

 1:250   1:150  

PROTEIN ARRAY
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1200  

ANTIGEN INFORMATION
 
 

Antibody HPA006659

  

Antibody HPA050674

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 119   130  

Antigen sequence

 
LLLPRSHSDPGITTSSDTADFRDLYTKVLEEEAASVSSADTGLCSEACLF
RLARCPSPKLLRARSAEKRRPVPTFQKVPLPSGPAPAHSLGDLKGSWPGR
GLVTRFLQISRKAPDPSGT
  
PLYPTDCPPSYEAVMGLRGDSQATLFDPQLHDGSCICERVASIVDVSMDS
GSLVLSAIGDLPGGSSPSEDSCLLELQGSVRSVDYVLFRSIQRSRAGYCL
SLDCGLRGPFEESPLPRRPPRAARSYSCSA
 

Matching transcripts

 FAM189B-001 - ENSP00000354958 [100%]
FAM189B-002 - ENSP00000307128 [100%]
FAM189B-004 - ENSP00000357352 [100%]
FAM189B-008 - ENSP00000427011 [84%]
  FAM189B-001 - ENSP00000354958 [100%]
FAM189B-002 - ENSP00000307128 [100%]
FAM189B-004 - ENSP00000357352 [100%]
 

Other gene match

    
ANTIGEN VIEW
FAM189B-001
FAM189B-002
FAM189B-004
FAM189B-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.