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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes
Immunohistochemical staining of human gall bladder shows strong cytoplasmic and membrane positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 26 tissues Protein: detected in 52 cell types
RNA: detected in 26 tissues Protein: detected in 75 cell types
RNA: detected in 26 tissues Protein: detected in 31 cell types
RNA: detected in 26 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:75
1:75
1:35
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027273
Antibody HPA027369
Antibody HPA028251
Antibody CAB005196
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400735)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
88, 83.5, 80.5
88, 83.5, 80.5, 22.2
88, 83.5, 80.5, 22.2, 12.4, 11.8, 9.5
88, 83.5, 80.5, 30.3, 22.2, 12.4, 11.8, 9.5
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA027273
Antibody HPA027369
Antibody HPA028251
Antibody CAB005196
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
