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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:42
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021320
Antibody HPA029916
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
Antibody dilution
1:125
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021320
Antibody HPA029916
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407257)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407257)
Target mass (kDa)
70.9
70.9, 26.3
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA021320
Antibody HPA029916
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
