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Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human spleen showsstrong cytoplasmic positivity in a subset of cells in red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.
Expression
RNA: detected in 13 tissues Protein: detected in 43 cell types
RNA: detected in 13 tissues Protein: detected in 30 cell types
RNA: detected in 13 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:700
1:500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013859
Antibody CAB004962
Antibody CAB004963
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400456)
Target mass (kDa)
74.8, 70.1
74.8, 70.1, 12.3, 0.3
74.8, 70.1, 12.3, 0.3
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA013859
Antibody CAB004962
Antibody CAB004963
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
