Fields »
ABOUT
HELP
BLOG
MSX1

ANTIBODY INFORMATION
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA063895   HPA073604   AF5045  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 16   16   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA063895, RRID:AB_2685145   Atlas Antibodies Cat#HPA073604, RRID:AB_2686617    

VALIDATION SUMMARY
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.      

Antibody dilution

 1:21      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human endometrium shows nuclear positivity in glandular cells.   Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular cells.  

Expression

   RNA: detected in 22 tissues
Protein: detected in 10 cell types		   RNA: detected in 22 tissues
Protein: detected in 3 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:500   1:4000  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 31.5   31.5   31.5  

Antibody dilution

 1:80   1:300   1:500  

PROTEIN ARRAY
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:550   1:3550    

ANTIGEN INFORMATION
 
 

Antibody HPA063895

  

Antibody HPA073604

  

Antibody CAB026198

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 24   29    

Antigen sequence

 
MAPAADMTSLPLGVKVEDSAFGKP
  
LGHFSVGGLLKLPEDALVKAESPEKPERT
  

											 
																				

																		

										
											
Matching transcripts

												
																					MSX1-001 - ENSP00000372170 [100%] 

											 
																						MSX1-001 - ENSP00000372170 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MSX1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.