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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in lymphoid cells outside reaction centra.
Expression
RNA: detected in 25 tissues Protein: detected in 4 cell types
RNA: detected in 25 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:400
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002881
Antibody CAB025872
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723)
Target mass (kDa)
10.6
10.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002881
Antibody CAB025872
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
