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CLDN1

ANTIBODY INFORMATION
 
 

Antibody HPA048319

  

Antibody CAB002602

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA048319   RB-9209  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 13   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA048319, RRID:AB_2680355    

VALIDATION SUMMARY
 
 

Antibody HPA048319

  

Antibody CAB002602

  
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048319

  

Antibody CAB002602

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows distinct membranous and cytoplasmic positivity in hepatocytes and bile duct cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 36 cell types		   RNA: detected in 31 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:30   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048319

  

Antibody CAB002602

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 22.7   22.7  

Antibody dilution

 1:240   1:500  

PROTEIN ARRAY
 
 

Antibody HPA048319

  

Antibody CAB002602

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1950    

ANTIGEN INFORMATION
 
 

Antibody HPA048319

  

Antibody CAB002602

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 23    

Antigen sequence

 
KTTSYPTPRPYPKPAPSSGKDYV
  

											 
																				

																		

										
											
Matching transcripts

												
																					CLDN1-001 - ENSP00000295522 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CLDN1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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