YY1AP1 - Antibodies - The Human Protein Atlas

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YY1AP1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006986

Antibody HPA064249

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA006986

HPA064249

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

GON4L - ENSG00000116580 [88%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006986, RRID:AB_1080620

Atlas Antibodies Cat#HPA064249, RRID:AB_2685226

VALIDATION SUMMARY

Antibody HPA006986

Antibody HPA064249

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006986

Antibody HPA064249

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.

Antibody dilution

1:28

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006986

Antibody HPA064249

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:15

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006986

Antibody HPA064249

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

248.6, 248.5, 170.7, 169.1, 109.8, 97.7, 95.5, 87.9, 83.1, 81.7, 80.9, 79.5, 55.8, 23.6, 22.4

97.7, 95.5, 87.9, 55.8

Antibody dilution

1:250

1:90

PROTEIN ARRAY

Antibody HPA006986

Antibody HPA064249

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1000

ANTIGEN INFORMATION

Antibody HPA006986

Antibody HPA064249

Antigen

Recombinant protein fragment

Length (aa)

133

Antigen sequence

MEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLK
MKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQI
HLLATCNPNLNPEASSTRICLKELGTFAQSSIA

TNPGSRLTRWPPPDKREGSAVDPGKRRSLAATPSSSLPCTLIALGLRHEK
EANELMED

Matching transcripts

YY1AP1-024 - ENSP00000357324 [100%]
YY1AP1-025 - ENSP00000355298 [100%]
YY1AP1-027 - ENSP00000352134 [100%]
YY1AP1-032 - ENSP00000311138 [100%]
YY1AP1-034 - ENSP00000347686 [100%]
YY1AP1-035 - ENSP00000409203 [100%]
YY1AP1-040 - ENSP00000316079 [100%]
YY1AP1-041 - ENSP00000413240 [100%]
YY1AP1-042 - ENSP00000384583 [100%]
YY1AP1-043 - ENSP00000295566 [100%]
YY1AP1-044 - ENSP00000357314 [100%]
YY1AP1-045 - ENSP00000385791 [100%]
YY1AP1-046 - ENSP00000385390 [100%]
YY1AP1-201 - ENSP00000357323 [100%]

YY1AP1-043 - ENSP00000295566 [100%]
YY1AP1-024 - ENSP00000357324 [93%]
YY1AP1-042 - ENSP00000384583 [93%]
YY1AP1-201 - ENSP00000357323 [93%]

Other gene match

GON4L - ENSG00000116580 [88%]

ANTIGEN VIEW

YY1AP1-024	YY1AP1-025	YY1AP1-027	YY1AP1-032	YY1AP1-034	YY1AP1-035	YY1AP1-040	YY1AP1-041	YY1AP1-042	YY1AP1-043	YY1AP1-044	YY1AP1-045	YY1AP1-046	YY1AP1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.