GON4L - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

GON4L

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA006986

HPA057305

HPA074504

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

YY1AP1 - ENSG00000163374 [100%]

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006986, RRID:AB_1080620

Atlas Antibodies Cat#HPA057305, RRID:AB_2683408

Atlas Antibodies Cat#HPA074504, RRID:AB_2686696

VALIDATION SUMMARY

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA074504.

Validated

Antibody staining overlaps with antibody HPA057305.

Standard validation

Approved

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nuclear bodies.

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:83

1:75

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:15

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

248.6, 248.5, 170.7, 169.1, 109.8, 97.7, 95.5, 87.9, 83.1, 81.7, 80.9, 79.5, 55.8, 23.6, 22.4

248.6, 248.5, 170.7, 169.1, 109.8

Antibody dilution

1:250

1:270

PROTEIN ARRAY

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3300

1:3000

ANTIGEN INFORMATION

Antibody HPA006986

Antibody HPA057305

Antibody HPA074504

Antigen

Recombinant protein fragment

Length (aa)

133

104

Antigen sequence

MEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLK
MKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQI
HLLATCNPNLNPEASSTRICLKELGTFAQSSIA

PMEEQDNEESEKRRKKKKGTKRKRDGRGQEGTLAYDLKLDDMLDRTLEDG
AKQHNLTAVNVRNILHEVITNEHVVAMMKAAISETEDMPMFEPKMTRSKL
KEVV

PVCMDSFQPMDDSLIAFQTRSKMPLKDVPLGQLEAELQAPDITPDMYDPN
TADDEDWKMWLGGLMNDDVGNEDE

Matching transcripts

GON4L-001 - ENSP00000354322 [88%]
GON4L-018 - ENSP00000271883 [88%]
GON4L-019 - ENSP00000396117 [88%]
GON4L-201 - ENSP00000357315 [88%]
GON4L-202 - ENSP00000483100 [88%]
GON4L-203 - ENSP00000481460 [88%]
GON4L-204 - ENSP00000478593 [88%]

GON4L-001 - ENSP00000354322 [100%]
GON4L-018 - ENSP00000271883 [100%]
GON4L-019 - ENSP00000396117 [100%]
GON4L-201 - ENSP00000357315 [100%]
GON4L-202 - ENSP00000483100 [100%]
GON4L-203 - ENSP00000481460 [100%]
GON4L-204 - ENSP00000478593 [100%]

GON4L-001 - ENSP00000354322 [99%]
GON4L-018 - ENSP00000271883 [99%]
GON4L-019 - ENSP00000396117 [99%]
GON4L-201 - ENSP00000357315 [99%]
GON4L-202 - ENSP00000483100 [99%]
GON4L-203 - ENSP00000481460 [99%]
GON4L-204 - ENSP00000478593 [99%]

Other gene match

YY1AP1 - ENSG00000163374 [100%]

ANTIGEN VIEW

GON4L-001	GON4L-018	GON4L-019	GON4L-201	GON4L-202	GON4L-203	GON4L-204

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.