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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PRKCI

Gene description

Protein kinase C, iota

Protein class

Cancer-related genes
Enzymes
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

1
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 1 antibody.
HPA026574
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PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (unfavourable), pancreatic cancer (unfavourable), liver cancer (unfavourable) and renal cancer (favourable).
Endometrial cancer p<0.001
Pancreatic cancer p<0.001
Liver cancer p<0.001
Renal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Ovarian cancer

STAINING SUMMARY

HPA026574

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several cancers displayed weak to moderate cytoplasmic positivity while ovarian cancers showed moderate to strong staining. Gliomas, lymphomas, melanomas, renal and liver cancers were generally negative.

GENE INFORMATION

Gene name

PRKCI (HGNC Symbol)

Synonyms

DXS1179E, PKCI

Description

Protein kinase C, iota (HGNC Symbol)

Entrez gene summary

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

Chromosome

3

Cytoband

q26.2

Chromosome location (bp)

170222365 - 170305981

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000163558 (version 83.38)

Entrez gene

5584

UniProt

P41743 (UniProt - Evidence at protein level)

neXtProt

NX_P41743

Antibodypedia

PRKCI antibodies

PROTEIN BROWSER

PRKCI-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PRKCI-001
ENSP00000295797
ENST00000295797
P41743 [Direct mapping]
Protein kinase C iota type
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   AGC Ser/Thr protein kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0004697 [protein kinase C activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005543 [phospholipid binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005768 [endosome]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005923 [bicellular tight junction]
GO:0006468 [protein phosphorylation]
GO:0006612 [protein targeting to membrane]
GO:0007010 [cytoskeleton organization]
GO:0007015 [actin filament organization]
GO:0010976 [positive regulation of neuron projection development]
GO:0015630 [microtubule cytoskeleton]
GO:0016192 [vesicle-mediated transport]
GO:0016324 [apical plasma membrane]
GO:0016477 [cell migration]
GO:0019904 [protein domain specific binding]
GO:0031252 [cell leading edge]
GO:0032869 [cellular response to insulin stimulus]
GO:0034329 [cell junction assembly]
GO:0034351 [negative regulation of glial cell apoptotic process]
GO:0034613 [cellular protein localization]
GO:0035089 [establishment of apical/basal cell polarity]
GO:0035556 [intracellular signal transduction]
GO:0042462 [eye photoreceptor cell development]
GO:0043066 [negative regulation of apoptotic process]
GO:0043220 [Schmidt-Lanterman incisure]
GO:0043234 [protein complex]
GO:0043434 [response to peptide hormone]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045171 [intercellular bridge]
GO:0045177 [apical part of cell]
GO:0045197 [establishment or maintenance of epithelial cell apical/basal polarity]
GO:0045216 [cell-cell junction organization]
GO:0046326 [positive regulation of glucose import]
GO:0046872 [metal ion binding]
GO:0046903 [secretion]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048194 [Golgi vesicle budding]
GO:0051092 [positive regulation of NF-kappaB transcription factor activity]
GO:0060252 [positive regulation of glial cell proliferation]
GO:0061024 [membrane organization]
GO:0070062 [extracellular exosome]
GO:0070555 [response to interleukin-1]
GO:0070830 [bicellular tight junction assembly]
GO:0090004 [positive regulation of establishment of protein localization to plasma membrane]
GO:2000353 [positive regulation of endothelial cell apoptotic process]
Show all
596 aa
68.3 kDa
No 0

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