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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line RT4 shows localization to mitochondria.
Antibody dilution
1:200
1:55
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031365
Antibody HPA040309
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 20 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031365
Antibody HPA040309
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417721)
Target mass (kDa)
40.4, 39, 19.1
40.4, 39
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA031365
Antibody HPA040309
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
