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KIAA1143

ANTIBODY INFORMATION
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045018   HPA061799  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045018, RRID:AB_10964578   Atlas Antibodies Cat#HPA061799, RRID:AB_2684614  

VALIDATION SUMMARY
 
 

Antibody HPA045018

  

Antibody HPA061799

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Supported  

Validated
  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to nucleus & cytokinetic bridge.  

Antibody dilution

   1:72  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 52 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1200    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

   17.5  

Loading control

   Total protein image  

Antibody dilution

   1:161  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 17.5   17.5  

Antibody dilution

 1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:2900  

ANTIGEN INFORMATION
 
 

Antibody HPA045018

  

Antibody HPA061799

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 58   42  

Antigen sequence

 
IKAEIKAAKADEEPTPADGRIIYRKPVKHPSDEKYSGLTASSKKKKPNED
EVNQDSVK
  
MSKRNQVSYVRPAEPAFLARFKERVGYREGPTVETKRIQPQP
 

Matching transcripts

 KIAA1143-001 - ENSP00000296121 [100%]
  KIAA1143-001 - ENSP00000296121 [100%]
 

Other gene match

    
ANTIGEN VIEW
KIAA1143-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.