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CDCP1

ANTIBODY INFORMATION
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA010978   HPA010979   MAB2666  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   4   6  

References

 1   1    

Proper citation

 Atlas Antibodies Cat#HPA010978, RRID:AB_1846403   Atlas Antibodies Cat#HPA010979, RRID:AB_1846401    

VALIDATION SUMMARY
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA010979 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA010978 across 72 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells and extracellular material of glandular lumina.   Immunohistochemical staining of human esophagus shows moderate membranous positivity.   Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 47 cell types		   RNA: detected in 29 tissues
Protein: detected in 43 cell types		   RNA: detected in 29 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:7   1:10   1:5000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 92.9   92.9, 37.8   92.9, 37.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA010978

  

Antibody HPA010979

  

Antibody CAB025637

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 119   147    

Antigen sequence

 
SKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVE
LHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQD
LYFGSFCPGGSIKQIQVKQ
  
GFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVP
AHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAG
NFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERA
  

											 
																				

																		

										
											
Matching transcripts

												
																					CDCP1-001 - ENSP00000296129 [100%] 

											 
																						CDCP1-001 - ENSP00000296129 [100%] 
CDCP1-002 - ENSP00000399342 [91%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDCP1-001
CDCP1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA010978

												
										 
																				
											
											
											
Antibody HPA010979

												
										 
																				
											
											
											
Antibody CAB025637

												
										 
																		

																	

										
											
1.

												
																						CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production

	Iwata M et al

	PLoS One 2014;9(10):e109304

			Application: IHC				
											 
																							CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production

	Iwata M et al

	PLoS One 2014;9(10):e109304

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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