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Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in endoplasmic reticulum & the Golgi apparatus and GFP expression in endoplasmic reticulum & the Golgi apparatus.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in endoplasmic reticulum & the Golgi apparatus and GFP expression in endoplasmic reticulum & the Golgi apparatus.
Antibody dilution
1:6
1:43
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA001890.
Validated
Antibody staining overlaps with antibody HPA001863.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:6
1:45
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001863
Antibody HPA001890
Antibody CAB010469
Antibody CAB013225
Formal validation: Orthogonal
Validated
Pearson correlation >0.6 for protein and RNA expression in cell lines.
Validated
Pearson correlation >0.6 for protein and RNA expression in cell lines.
Validated
Pearson correlation >0.6 for protein and RNA expression in cell lines.
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA001890 across 65 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA001863 across 65 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebellum shows strong positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 36 tissues Protein: detected in 63 cell types
RNA: detected in 36 tissues Protein: detected in 47 cell types
RNA: detected in 36 tissues Protein: detected in 60 cell types
RNA: detected in 36 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:10
1:100
1:1500
1:1000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001863
Antibody HPA001890
Antibody CAB010469
Antibody CAB013225
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403724)
Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77.5, 25.5, 14.2
77.5
77.5, 25.5, 14.2, 8.8
77.5, 25.5, 14.2, 8.8
Antibody dilution
1:250
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA001863
Antibody HPA001890
Antibody CAB010469
Antibody CAB013225
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
