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FBXW12

ANTIBODY INFORMATION
 
 

Antibody HPA037491

  

Antibody HPA037492

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037491   HPA037492  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA037491, RRID:AB_2675504   Atlas Antibodies Cat#HPA037492, RRID:AB_2675505  

VALIDATION SUMMARY
 
 

Antibody HPA037491

  

Antibody HPA037492

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037491

  

Antibody HPA037492

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in goblet cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 9 cell types		   RNA: detected in 2 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:750  

Literature conformity

 No avaliable gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037491

  

Antibody HPA037492

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 53.1, 50.8, 45, 34.5   53.1, 50.8, 34.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037491

  

Antibody HPA037492

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037491

  

Antibody HPA037492

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 93   91  

Antigen sequence

 
PQVFLTESLLRPSEGSVPLSTFLPHKLCASACWTPKVKNRITLMSQSSTG
KKTEFITFDLTTKKTGGQTVIQAYEIASFQVAAHLKCPIWMGA
  
SPVQEFHFSNLVTLPQMHLAITMDWKKTIKVWNCQDRDALAVLPMPQPCY
CMEAYLTKDGPFLMVGDAAGDIYTFTLPGLRDVSKVTAFQY
 

Matching transcripts

 FBXW12-001 - ENSP00000296438 [100%]
FBXW12-002 - ENSP00000413866 [100%]
FBXW12-003 - ENSP00000406139 [100%]
FBXW12-004 - ENSP00000414683 [100%]
  FBXW12-001 - ENSP00000296438 [99%]
FBXW12-003 - ENSP00000406139 [99%]
FBXW12-002 - ENSP00000413866 [81%]
 

Other gene match

    
ANTIGEN VIEW
FBXW12-001
FBXW12-002
FBXW12-003
FBXW12-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.