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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:128
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA007657
Antibody HPA008180
Antibody CAB008972
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 26 tissues Protein: detected in 60 cell types
RNA: detected in 26 tissues Protein: detected in 64 cell types
RNA: detected in 26 tissues Protein: detected in 81 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:50
1:350
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007657
Antibody HPA008180
Antibody CAB008972
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419321)
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Placenta Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
152.2, 147.2, 140.7
152.2, 147.2, 140.7
152.2, 147.2, 140.7, 62.3, 32.3, 30, 29.4
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA007657
Antibody HPA008180
Antibody CAB008972
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
