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Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044405
Antibody HPA050733
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in fraction of tubular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
Antibody dilution
1:175
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044405
Antibody HPA050733
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407466)
Target mass (kDa)
46.3, 44.7
46.3
Antibody dilution
1:250
1:120
PROTEIN ARRAY
Antibody HPA044405
Antibody HPA050733
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
