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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.
Expression
RNA: detected in 8 tissues Protein: detected in 5 cell types
RNA: detected in 8 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:5000
1:60000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015775
Antibody CAB069919
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412137)
Target mass (kDa)
10.1
10.1
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA015775
Antibody CAB069919
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
