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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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NOS3
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    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

NOS3

Gene description

Nitric oxide synthase 3 (endothelial cell)

Protein class

Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (BEWO, HUVEC TERT2, TIME)
HPA (normal tissue):Tissue enriched (spleen)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in endothelial cells, most abundant in spleen

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Antibody staining in cells/structures not annotated, view images.

Reliability score

Supported based on 1 antibody.
CAB002168
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable).
0.00.10.20.30.40.50.60.70.80.91.0 0 1 2 3 4 5 6 7 8 910111213141516 Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all
0.050100150200250300350FPKMBreast cancer (BRCA)Cervical cancer (CESC)Colorectal cancer (COAD,READ)Endometrial cancer (UCEC)Glioma (GBM)Head and neck cancer (HNSC)Liver cancer (LIHC)Lung cancer (LUAD,LUSC)Melanoma (SKCM)Ovarian cancer (OV)Pancreatic cancer (PAAD)Prostate cancer (PRAD)Renal cancer (KICH,KIRC,KIRP)Stomach cancer (STAD)Testis cancer (TGCT)Thyroid cancer (THCA)Urothelial cancer (BLCA)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

CAB002168

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Malignant tissues were in general negative. Distinct staining was observed in endothelial cells of tumor stroma.

GENE INFORMATION

Gene name

NOS3 (HGNC Symbol)

Synonyms

ECNOS, eNOS

Description

Nitric oxide synthase 3 (endothelial cell) (HGNC Symbol)

Entrez gene summary

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Chromosome

7

Cytoband

q36.1

Chromosome location (bp)

150990995 - 151014588

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000164867 (version 83.38)

Entrez gene

4846

UniProt

P29474 (UniProt - Evidence at protein level)

neXtProt

NX_P29474

Antibodypedia

NOS3 antibodies

PROTEIN BROWSER

NOS3-001
NOS3-002
NOS3-004
NOS3-005
NOS3-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

NOS3-001
ENSP00000297494
ENST00000297494
P29474 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001525 [angiogenesis]
GO:0001542 [ovulation from ovarian follicle]
GO:0001701 [in utero embryonic development]
GO:0001974 [blood vessel remodeling]
GO:0002028 [regulation of sodium ion transport]
GO:0003057 [regulation of the force of heart contraction by chemical signal]
GO:0003100 [regulation of systemic arterial blood pressure by endothelin]
GO:0003785 [actin monomer binding]
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005515 [protein binding]
GO:0005516 [calmodulin binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005886 [plasma membrane]
GO:0005901 [caveola]
GO:0006527 [arginine catabolic process]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007005 [mitochondrion organization]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0007596 [blood coagulation]
GO:0008217 [regulation of blood pressure]
GO:0008285 [negative regulation of cell proliferation]
GO:0009408 [response to heat]
GO:0010181 [FMN binding]
GO:0010544 [negative regulation of platelet activation]
GO:0014740 [negative regulation of muscle hyperplasia]
GO:0014806 [smooth muscle hyperplasia]
GO:0016491 [oxidoreductase activity]
GO:0019430 [removal of superoxide radicals]
GO:0020037 [heme binding]
GO:0030324 [lung development]
GO:0030666 [endocytic vesicle membrane]
GO:0031284 [positive regulation of guanylate cyclase activity]
GO:0031663 [lipopolysaccharide-mediated signaling pathway]
GO:0034405 [response to fluid shear stress]
GO:0034617 [tetrahydrobiopterin binding]
GO:0034618 [arginine binding]
GO:0043267 [negative regulation of potassium ion transport]
GO:0043542 [endothelial cell migration]
GO:0044281 [small molecule metabolic process]
GO:0045766 [positive regulation of angiogenesis]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0046209 [nitric oxide metabolic process]
GO:0046870 [cadmium ion binding]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0050880 [regulation of blood vessel size]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0051346 [negative regulation of hydrolase activity]
GO:0051926 [negative regulation of calcium ion transport]
GO:0055114 [oxidation-reduction process]
GO:0090382 [phagosome maturation]
GO:0097110 [scaffold protein binding]
GO:1902042 [negative regulation of extrinsic apoptotic signaling pathway via death domain receptors]
Show all
1203 aa
133.3 kDa
No 0
NOS3-002
ENSP00000417143
ENST00000461406
E7ESA7 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
   SCAMPI predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005516 [calmodulin binding]
GO:0005829 [cytosol]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0010181 [FMN binding]
GO:0016491 [oxidoreductase activity]
GO:0020037 [heme binding]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0055114 [oxidation-reduction process]
Show all
997 aa
111.1 kDa
No 0
NOS3-004
ENSP00000420215
ENST00000484524
P29474 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001974 [blood vessel remodeling]
GO:0003100 [regulation of systemic arterial blood pressure by endothelin]
GO:0003785 [actin monomer binding]
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005515 [protein binding]
GO:0005516 [calmodulin binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005886 [plasma membrane]
GO:0005901 [caveola]
GO:0006527 [arginine catabolic process]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007005 [mitochondrion organization]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0007596 [blood coagulation]
GO:0008217 [regulation of blood pressure]
GO:0008285 [negative regulation of cell proliferation]
GO:0009408 [response to heat]
GO:0010181 [FMN binding]
GO:0010544 [negative regulation of platelet activation]
GO:0014740 [negative regulation of muscle hyperplasia]
GO:0014806 [smooth muscle hyperplasia]
GO:0019430 [removal of superoxide radicals]
GO:0020037 [heme binding]
GO:0030666 [endocytic vesicle membrane]
GO:0031284 [positive regulation of guanylate cyclase activity]
GO:0034405 [response to fluid shear stress]
GO:0034617 [tetrahydrobiopterin binding]
GO:0034618 [arginine binding]
GO:0043542 [endothelial cell migration]
GO:0044281 [small molecule metabolic process]
GO:0045766 [positive regulation of angiogenesis]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0046209 [nitric oxide metabolic process]
GO:0046870 [cadmium ion binding]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0050880 [regulation of blood vessel size]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0055114 [oxidation-reduction process]
GO:0090382 [phagosome maturation]
GO:1902042 [negative regulation of extrinsic apoptotic signaling pathway via death domain receptors]
Show all
629 aa
69 kDa
No 0
NOS3-005
ENSP00000420551
ENST00000467517
A0A0A0MTA6 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005829 [cytosol]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0010181 [FMN binding]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0055114 [oxidation-reduction process]
Show all
614 aa
67.9 kDa
No 0
NOS3-006
ENSP00000418245
ENST00000475017
H7C4V4 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0005829 [cytosol]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0016491 [oxidoreductase activity]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0055114 [oxidation-reduction process]
Show all
306 aa
33.8 kDa
No 0

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The Human Protein Atlas project is funded
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