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NOS3
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    TISSUE

  • CELL

  • PATHOLOGY

TISSUE ATLAS PRIMARY DATA

GENE/PROTEIN

Antibody validation


Dictionary


Tissue proteome

GENERAL INFORMATION

Gene name

NOS3

Gene description

Nitric oxide synthase 3 (endothelial cell)

Protein class

Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA tissue category

HPA:Tissue enriched (spleen)
GTEx:Expressed in all
FANTOM5:Expressed in all

Protein evidence

Evidence at protein level

Protein expression

Cytoplasmic expression in endothelial cells, most abundant in spleen

DATA RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Antibody staining in cells/structures not annotated, view images.

Reliability score

Supported based on 1 antibody.
CAB002168
SHOW MORE

RNA AND PROTEIN EXPRESSION SUMMARY

RNA expression (TPM) Protein expression (score)
Brain
Cerebral cortex
Hippocampus
N/A
Caudate
N/A
Cerebellum
N/A
Endocrine tissues
Thyroid gland
Parathyroid gland
Adrenal gland
Bone marrow & immune system
Appendix
Bone marrow
Lymph node
Tonsil
Spleen
Muscle tissues
Heart muscle
Skeletal muscle
Smooth muscle
Lung
Nasopharynx
N/A
Bronchus
N/A
Lung
Liver & gallbladder
Liver
Gallbladder
Pancreas
Pancreas
Gastrointestinal tract
Oral mucosa
N/A
Salivary gland
Esophagus
Stomach
Duodenum
Small intestine
Colon
Rectum
Kidney & urinary bladder
Kidney
Urinary bladder
Male tissues
Testis
Epididymis
Prostate
Seminal vesicle
Female tissues
Breast
Vagina
N/A
Cervix, uterine
Endometrium
Fallopian tube
Ovary
Placenta
Adipose & soft tissue
Adipose tissue
Soft tissue
N/A
Skin
Skin
Cerebral cortex

Lymph node

Spleen

Liver

Colon

Kidney

Testis

PROTEIN EXPRESSION OVERVIEW

Organ
Expression
Alphabetical
Cerebral cortexHippocampusCaudateCerebellumThyroid glandParathyroid glandAdrenal glandAppendixBone marrowLymph nodeTonsilSpleenHeart muscleSkeletal muscleSmooth muscleNasopharynxBronchusLungLiverGallbladderPancreasOral mucosaSalivary glandEsophagusStomachDuodenumSmall intestineColonRectumKidneyUrinary bladderTestisEpididymisProstateSeminal vesicleBreastVaginaCervix, uterineEndometriumFallopian tubeOvaryPlacentaAdipose tissueSoft tissueSkinNotdetectedLowMediumHighSCORE

RNA EXPRESSION OVERVIEW

HPA dataset
Organ
Expression
Alphabetical
RNA tissue category: Tissue enriched (spleen)
Cerebral cortexThyroid glandParathyroid glandAdrenal glandAppendixBone marrowLymph nodeTonsilSpleenHeart muscleSkeletal muscleSmooth muscleLungLiverGallbladderPancreasSalivary glandEsophagusStomachDuodenumSmall intestineColonRectumKidneyUrinary bladderTestisEpididymisProstateSeminal vesicleBreastCervix, uterineEndometriumFallopian tubeOvaryPlacentaAdipose tissueSkin020406080TPM
GTEx dataset
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
HypothalamusPituitary glandCerebral cortexHippocampusCaudateCerebellumThyroid glandAdrenal glandSpleenHeart muscleSkeletal muscleLungLiverPancreasSalivary glandEsophagusStomachSmall intestineColonKidneyUrinary bladderTestisProstateBreastVaginaCervix, uterineEndometriumFallopian tubeOvaryAdipose tissueSkin05101520253035RPKM
FANTOM5 dataset
Organ
Expression
Alphabetical
RNA tissue category: Expressed in all
Pituitary glandBrainHippocampusCaudateCerebellumThyroid glandAppendixLymph nodeTonsilSpleenHeart muscleSkeletal muscleSmooth muscleLungLiverGallbladderPancreasSalivary glandEsophagusSmall intestineColonKidneyUrinary bladderTestisEpididymisProstateSeminal vesicleBreastVaginaCervix, uterineEndometriumOvaryPlacentaAdipose tissueRetina0100200300400500Tags Per Million

GENE INFORMATION

Gene name

NOS3 (HGNC Symbol)

Synonyms

ECNOS, eNOS

Description

Nitric oxide synthase 3 (endothelial cell) (HGNC Symbol)

Entrez gene summary

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Chromosome

7

Cytoband

q36.1

Chromosome location (bp)

150990995 - 151014588

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000164867 (version 83.38)

Entrez gene

4846

UniProt

P29474 (UniProt - Evidence at protein level)

neXtProt

NX_P29474

Antibodypedia

NOS3 antibodies

PROTEIN BROWSER

NOS3-001
NOS3-002
NOS3-004
NOS3-005
NOS3-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

NOS3-001
ENSP00000297494
ENST00000297494
P29474 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001525 [angiogenesis]
GO:0001542 [ovulation from ovarian follicle]
GO:0001701 [in utero embryonic development]
GO:0001974 [blood vessel remodeling]
GO:0002028 [regulation of sodium ion transport]
GO:0003057 [regulation of the force of heart contraction by chemical signal]
GO:0003100 [regulation of systemic arterial blood pressure by endothelin]
GO:0003785 [actin monomer binding]
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005515 [protein binding]
GO:0005516 [calmodulin binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005886 [plasma membrane]
GO:0005901 [caveola]
GO:0006527 [arginine catabolic process]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007005 [mitochondrion organization]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0007596 [blood coagulation]
GO:0008217 [regulation of blood pressure]
GO:0008285 [negative regulation of cell proliferation]
GO:0009408 [response to heat]
GO:0010181 [FMN binding]
GO:0010544 [negative regulation of platelet activation]
GO:0014740 [negative regulation of muscle hyperplasia]
GO:0014806 [smooth muscle hyperplasia]
GO:0016491 [oxidoreductase activity]
GO:0019430 [removal of superoxide radicals]
GO:0020037 [heme binding]
GO:0030324 [lung development]
GO:0030666 [endocytic vesicle membrane]
GO:0031284 [positive regulation of guanylate cyclase activity]
GO:0031663 [lipopolysaccharide-mediated signaling pathway]
GO:0034405 [response to fluid shear stress]
GO:0034617 [tetrahydrobiopterin binding]
GO:0034618 [arginine binding]
GO:0043267 [negative regulation of potassium ion transport]
GO:0043542 [endothelial cell migration]
GO:0044281 [small molecule metabolic process]
GO:0045766 [positive regulation of angiogenesis]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0046209 [nitric oxide metabolic process]
GO:0046870 [cadmium ion binding]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0050880 [regulation of blood vessel size]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0051346 [negative regulation of hydrolase activity]
GO:0051926 [negative regulation of calcium ion transport]
GO:0055114 [oxidation-reduction process]
GO:0090382 [phagosome maturation]
GO:0097110 [scaffold protein binding]
GO:1902042 [negative regulation of extrinsic apoptotic signaling pathway via death domain receptors]
Show all
1203 aa
133.3 kDa
No 0
NOS3-002
ENSP00000417143
ENST00000461406
E7ESA7 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
   SCAMPI predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005516 [calmodulin binding]
GO:0005829 [cytosol]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0010181 [FMN binding]
GO:0016491 [oxidoreductase activity]
GO:0020037 [heme binding]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0055114 [oxidation-reduction process]
Show all
997 aa
111.1 kDa
No 0
NOS3-004
ENSP00000420215
ENST00000484524
P29474 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0001974 [blood vessel remodeling]
GO:0003100 [regulation of systemic arterial blood pressure by endothelin]
GO:0003785 [actin monomer binding]
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005515 [protein binding]
GO:0005516 [calmodulin binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005886 [plasma membrane]
GO:0005901 [caveola]
GO:0006527 [arginine catabolic process]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007005 [mitochondrion organization]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0007596 [blood coagulation]
GO:0008217 [regulation of blood pressure]
GO:0008285 [negative regulation of cell proliferation]
GO:0009408 [response to heat]
GO:0010181 [FMN binding]
GO:0010544 [negative regulation of platelet activation]
GO:0014740 [negative regulation of muscle hyperplasia]
GO:0014806 [smooth muscle hyperplasia]
GO:0019430 [removal of superoxide radicals]
GO:0020037 [heme binding]
GO:0030666 [endocytic vesicle membrane]
GO:0031284 [positive regulation of guanylate cyclase activity]
GO:0034405 [response to fluid shear stress]
GO:0034617 [tetrahydrobiopterin binding]
GO:0034618 [arginine binding]
GO:0043542 [endothelial cell migration]
GO:0044281 [small molecule metabolic process]
GO:0045766 [positive regulation of angiogenesis]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0046209 [nitric oxide metabolic process]
GO:0046870 [cadmium ion binding]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0050660 [flavin adenine dinucleotide binding]
GO:0050661 [NADP binding]
GO:0050880 [regulation of blood vessel size]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0055114 [oxidation-reduction process]
GO:0090382 [phagosome maturation]
GO:1902042 [negative regulation of extrinsic apoptotic signaling pathway via death domain receptors]
Show all
629 aa
69 kDa
No 0
NOS3-005
ENSP00000420551
ENST00000467517
A0A0A0MTA6 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0004517 [nitric-oxide synthase activity]
GO:0005506 [iron ion binding]
GO:0005829 [cytosol]
GO:0006809 [nitric oxide biosynthetic process]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0010181 [FMN binding]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0055114 [oxidation-reduction process]
Show all
614 aa
67.9 kDa
No 0
NOS3-006
ENSP00000418245
ENST00000475017
H7C4V4 [Direct mapping]
Nitric oxide synthase, endothelial
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003958 [NADPH-hemoprotein reductase activity]
GO:0005829 [cytosol]
GO:0007263 [nitric oxide mediated signal transduction]
GO:0016491 [oxidoreductase activity]
GO:0045776 [negative regulation of blood pressure]
GO:0045909 [positive regulation of vasodilation]
GO:0055114 [oxidation-reduction process]
Show all
306 aa
33.8 kDa
No 0

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