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Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & mitochondria.
Antibody dilution
1:15
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA031621
Antibody CAB032539
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:60
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031621
Antibody CAB032539
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.1, 58.2, 45.9
61.1, 58.2, 51.5, 45.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA031621
Antibody CAB032539
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
