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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
Antibody dilution
1:5
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA057283
Antibody CAB069889
Standard validation
Approved
Figure description
Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in macrophages.
Expression
RNA: detected in 36 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
Antibody dilution
1:900
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA057283
Antibody CAB069889
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
254.3, 41.1
254.3, 41.1, 13.8
Antibody dilution
1:80
1:500
PROTEIN ARRAY
Antibody HPA057283
Antibody CAB069889
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
