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WNK2

ANTIBODY INFORMATION
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA015555   HPA016519   2591.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   12   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA015555, RRID:AB_2669218   Atlas Antibodies Cat#HPA016519, RRID:AB_2669380    

VALIDATION SUMMARY
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated

Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.      

Antibody dilution

 1:125      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA016519 across 71 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA015555 across 71 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 30 tissues
Protein: detected in 47 cell types		   RNA: detected in 30 tissues
Protein: detected in 63 cell types		   RNA: detected in 30 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:175   1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 242.7, 234.5, 234.4, 185.8, 106.1, 67.4, 38.6   242.7, 234.5, 234.4, 185.8, 106.1, 67.4, 38.6   242.7, 234.5, 234.4, 185.8, 106.1, 85.9, 67.4, 38.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA015555

  

Antibody HPA016519

  

Antibody CAB017813

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 72   128    

Antigen sequence

 
KHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKS
KLKAGKLLNPLVRQLKVVASST
  
GTSSSMTAESSPRSMLGYDRDGRQVASDSHVVPSVPQDVPAFVRPARVEP
TDRDGGEAGESSAEPPPSDMGTVGGQASHPQTLGARALGSPRKRPEQQDV
SSPAKTVGRFSVVSTQDEWTLASPHSLR
   

Matching transcripts

 WNK2-001 - ENSP00000297954 [100%]
WNK2-002 - ENSP00000390441 [100%]
WNK2-009 - ENSP00000415038 [100%]
WNK2-010 - ENSP00000414622 [100%]
WNK2-014 - ENSP00000413325 [100%]
WNK2-201 - ENSP00000378860 [100%]
WNK2-202 - ENSP00000411181 [100%]
  WNK2-001 - ENSP00000297954 [100%]
WNK2-002 - ENSP00000390441 [100%]
WNK2-009 - ENSP00000415038 [100%]
WNK2-010 - ENSP00000414622 [100%]
WNK2-201 - ENSP00000378860 [100%]
WNK2-014 - ENSP00000413325 [99%]
WNK2-202 - ENSP00000411181 [99%]
   

Other gene match

      
ANTIGEN VIEW
WNK2-001
WNK2-002
WNK2-009
WNK2-010
WNK2-014
WNK2-201
WNK2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.