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CLDN2

ANTIBODY INFORMATION
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA051548   RB-9260  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 12   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051548, RRID:AB_2681530    

VALIDATION SUMMARY
 
 

Antibody HPA051548

  

Antibody CAB002609

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.    

Antibody dilution

 1:66    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.  

Expression

   RNA: detected in 14 tissues
Protein: detected in 26 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:400  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 24.5   24.5  

Antibody dilution

 1:330   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2650    

ANTIGEN INFORMATION
 
 

Antibody HPA051548

  

Antibody CAB002609

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 40    

Antigen sequence

 
RSNYYDAYQAQPLATRSSPRPGQPPKVKSEFNSYSLTGYV
  

											 
																				

																		

										
											
Matching transcripts

												
																					CLDN2-001 - ENSP00000336571 [100%] 
CLDN2-201 - ENSP00000443230 [100%] 
CLDN2-202 - ENSP00000441283 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CLDN2-001
CLDN2-201
CLDN2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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