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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:137
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035360
Antibody HPA038713
Antibody HPA052451
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong positivity in a subset of cells.
Immunohistochemical staining of human duodenum shows strong positivity in a subset of glandular cells.
Expression
RNA: detected in 25 tissues Protein: detected in 80 cell types
RNA: detected in 25 tissues Protein: detected in 19 cell types
RNA: detected in 25 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:1500
1:350
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035360
Antibody HPA038713
Antibody HPA052451
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407941)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407941)
Target mass (kDa)
35.2, 29.4, 28.3
35.2, 17.2, 16.3
35.2, 17.2, 16.3
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA035360
Antibody HPA038713
Antibody HPA052451
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
