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Antibody staining overlaps with antibody HPA011152.
Validated
Antibody staining overlaps with antibody HPA011122.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear speckles.
Antibody dilution
1:105
1:105
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA011122
Antibody HPA011152
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferous ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 4 cell types
RNA: detected in 1 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011122
Antibody HPA011152
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403556)
Target mass (kDa)
87.4
87.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA011122
Antibody HPA011152
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
