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NOLC1

ANTIBODY INFORMATION
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA037366   HPA050388   sc-28672  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 7   12   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA037366, RRID:AB_10670930   Atlas Antibodies Cat#HPA050388, RRID:AB_2681109   Santa Cruz Biotechnology Cat#sc-28672, RRID:AB_2152006  

VALIDATION SUMMARY
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA050388.		  
Validated

Antibody staining overlaps with antibody HPA037366.		    
 

Standard validation

 Supported   Uncertain    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.   Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.    

Antibody dilution

 1:34   1:42    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.   Immunohistochemical staining of human esophagus shows moderate nucleolar positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 32 cell types		   RNA: detected in 37 tissues
Protein: detected in 31 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:850   1:850  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 74.7, 74.5, 73.7, 73.6   74.7, 74.5, 73.7, 73.6   74.7, 74.5, 73.7, 73.6, 29.2, 26.8  

Antibody dilution

 1:250   1:210   1:500  

PROTEIN ARRAY
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1700    

ANTIGEN INFORMATION
 
 

Antibody HPA037366

  

Antibody HPA050388

  

Antibody CAB032654

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 72   90    

Antigen sequence

 
VPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLK
SAKVPERKLQANGPVAKKAKKK
  
VVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSP
FRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKF
  

											 
																				

																		

										
											
Matching transcripts

												
																					NOLC1-001 - ENSP00000474710 [100%] 
NOLC1-008 - ENSP00000359024 [100%] 
NOLC1-201 - ENSP00000385410 [100%] 
NOLC1-009 - ENSP00000475080 [97%] 

											 
																						NOLC1-001 - ENSP00000474710 [100%] 
NOLC1-008 - ENSP00000359024 [100%] 
NOLC1-009 - ENSP00000475080 [100%] 
NOLC1-201 - ENSP00000385410 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NOLC1-001
NOLC1-008
NOLC1-009
NOLC1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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