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PPFIBP2

ANTIBODY INFORMATION
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001935   HPA003911  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA001935, RRID:AB_1079667   Atlas Antibodies Cat#HPA003911, RRID:AB_1079666  

VALIDATION SUMMARY
 
 

Antibody HPA001935

  

Antibody HPA003911

  
Immunocytochemistry

Approved    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.    

Antibody dilution

 1:30    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA003911 across 70 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA001935 across 70 cell types.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 98.5, 86.5, 83.2, 81.4, 24.1   98.5, 86.5, 83.2, 81.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001935

  

Antibody HPA003911

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   119  

Antigen sequence

 
SGATPNGEAAKSPPTICQPDATGSSLLRLRDTESGWDDTAVVNDLSSTSS
GTESGPQSPLTPDGKRNPKGIKKFWGKIRRTQSGNFYTDTLGMAEFRRGG
L
  
TAALHSESHTERDQEIQRLKMGMETLLLANEDKDRRIEELTGLLNQYRKV
KEIVMVTQGPSERTLSINEEEPEGGFSKWNATNKDPEELFKQEMPPRCSS
PTVGPPPLPQKSLETRAQK
 

Matching transcripts

 PPFIBP2-001 - ENSP00000299492 [100%]
PPFIBP2-003 - ENSP00000436498 [100%]
PPFIBP2-007 - ENSP00000435469 [100%]
PPFIBP2-008 - ENSP00000437321 [100%]
PPFIBP2-022 - ENSP00000433713 [89%]
  PPFIBP2-001 - ENSP00000299492 [100%]
PPFIBP2-003 - ENSP00000436498 [100%]
PPFIBP2-007 - ENSP00000435469 [100%]
PPFIBP2-008 - ENSP00000437321 [100%]
 

Other gene match

    
ANTIGEN VIEW
PPFIBP2-001
PPFIBP2-003
PPFIBP2-007
PPFIBP2-008
PPFIBP2-022
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.