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WDR72

ANTIBODY INFORMATION
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048212   HPA057410   HPA059819  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   12   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA048212, RRID:AB_2680315   Atlas Antibodies Cat#HPA057410, RRID:AB_2683430   Atlas Antibodies Cat#HPA059819, RRID:AB_2684133  

VALIDATION SUMMARY
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  
Immunocytochemistry

Approved      
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to vesicles.      

Antibody dilution

 1:32      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human kidney shows moderate nuclear and membranous positivity in cells in tubules.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.  

Expression

   RNA: detected in 19 tissues
Protein: detected in 38 cell types		   RNA: detected in 19 tissues
Protein: detected in 35 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:1100   1:2000  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 124.6, 123.4, 123, 86.8   124.6, 123.4, 123, 86.8   124.6, 123.4, 123  

Antibody dilution

 1:160   1:610   1:480  

PROTEIN ARRAY
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1250   1:7250   1:5750  

ANTIGEN INFORMATION
 
 

Antibody HPA048212

  

Antibody HPA057410

  

Antibody HPA059819

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 120   120   96  

Antigen sequence

 
GHSYIYQLLNSGLSKSIYPADGRVLKETIYPHLLCSTSVQENKEQSRPFV
MGYMNERKEPFYKVLFSGEVSGRITLWHIPDVPVSKFDGSPREIPVTATW
TLQDNFDKHDTMSQSIIDYF
  
GHSYIYQLLNSGLSKSIYPADGRVLKETIYPHLLCSTSVQENKEQSRPFV
MGYMNERKEPFYKVLFSGEVSGRITLWHIPDVPVSKFDGSPREIPVTATW
TLQDNFDKHDTMSQSIIDYF
  
LISCWRDQSVQVTEAIQAVLLAEVQQHMKSLGKIPVNSQPVSMAENGNCE
MKQMLPKLEWTEELELQCVRNTLPLQTPVSPVKHDSNSNSANFQDV
 

Matching transcripts

 WDR72-001 - ENSP00000379619 [100%]
WDR72-002 - ENSP00000353699 [100%]
WDR72-004 - ENSP00000453813 [100%]
WDR72-003 - ENSP00000453378 [98%]
WDR72-005 - ENSP00000452765 [89%]
  WDR72-001 - ENSP00000379619 [100%]
WDR72-002 - ENSP00000353699 [100%]
WDR72-004 - ENSP00000453813 [100%]
WDR72-003 - ENSP00000453378 [98%]
WDR72-005 - ENSP00000452765 [89%]
  WDR72-001 - ENSP00000379619 [100%]
WDR72-002 - ENSP00000353699 [100%]
WDR72-003 - ENSP00000453378 [100%]
WDR72-005 - ENSP00000452765 [100%]
 

Other gene match

      
ANTIGEN VIEW
WDR72-001
WDR72-002
WDR72-003
WDR72-004
WDR72-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.