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PLK1

ANTIBODY INFORMATION
 
 

Antibody HPA051638

  

Antibody HPA053229

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051638   HPA053229  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA051638, RRID:AB_2681561   Atlas Antibodies Cat#HPA053229, RRID:AB_2682084  

VALIDATION SUMMARY
 
 

Antibody HPA051638

  

Antibody HPA053229

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051638

  

Antibody HPA053229

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA053229 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA051638 across 74 cell types.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 24 tissues
Protein: detected in 55 cell types		   RNA: detected in 24 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:30   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051638

  

Antibody HPA053229

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 68.3   68.3  

Antibody dilution

 1:80   1:170  

PROTEIN ARRAY
 
 

Antibody HPA051638

  

Antibody HPA053229

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1400  

ANTIGEN INFORMATION
 
 

Antibody HPA051638

  

Antibody HPA053229

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   87  

Antigen sequence

 
RPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTV
LNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNA
  
AKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVV
GFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEP
 

Matching transcripts

 PLK1-001 - ENSP00000300093 [100%]
  PLK1-001 - ENSP00000300093 [100%]
 

Other gene match

    
ANTIGEN VIEW
PLK1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.