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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PLK1
  • TISSUE

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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PLK1

Gene description

Polo-like kinase 1

Protein class

Cancer-related genes
Disease related genes
Enzymes
Potential drug targets
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Tissue enhanced (testis)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA051638 , HPA053229
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable), liver cancer (unfavourable), lung cancer (unfavourable) and pancreatic cancer (unfavourable).
Renal cancer p<0.001
Liver cancer p<0.001
Lung cancer p<0.001
Pancreatic cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA051638
HPA053229

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Weak to moderate nuclear membranous or nuclear positivity was observed in most cancer tissues. Prostate cancers displayed weak to moderate cytoplasmic immunoreactivity, with a granular pattern. Lymphomas, carcinoids, gliomas, colorectal, testicular and stomach cancers were generally negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues displayed moderate nuclear and cytoplasmic positivity. A few melanomas along with occasional adenocarcinomas of cervix and endometrial cancers were strongly stained.

GENE INFORMATION

Gene name

PLK1 (HGNC Symbol)

Synonyms

PLK

Description

Polo-like kinase 1 (HGNC Symbol)

Entrez gene summary

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Chromosome

16

Cytoband

p12.2

Chromosome location (bp)

23677656 - 23690367

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000166851 (version 83.38)

Entrez gene

5347

UniProt

P53350 (UniProt - Evidence at protein level)

neXtProt

NX_P53350

Antibodypedia

PLK1 antibodies

PROTEIN BROWSER

PLK1-001
PLK1-007
PLK1-008

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PLK1-001
ENSP00000300093
ENST00000300093
P53350 [Direct mapping]
Serine/threonine-protein kinase PLK1
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000070 [mitotic sister chromatid segregation]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0000278 [mitotic cell cycle]
GO:0000281 [mitotic cytokinesis]
GO:0000776 [kinetochore]
GO:0000780 [condensed nuclear chromosome, centromeric region]
GO:0000785 [chromatin]
GO:0000795 [synaptonemal complex]
GO:0000910 [cytokinesis]
GO:0000922 [spindle pole]
GO:0000942 [condensed nuclear chromosome outer kinetochore]
GO:0001578 [microtubule bundle formation]
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005819 [spindle]
GO:0005829 [cytosol]
GO:0005876 [spindle microtubule]
GO:0006468 [protein phosphorylation]
GO:0006996 [organelle organization]
GO:0007067 [mitotic nuclear division]
GO:0007077 [mitotic nuclear envelope disassembly]
GO:0007091 [metaphase/anaphase transition of mitotic cell cycle]
GO:0007092 [activation of APC-Cdc20 complex activity]
GO:0007094 [mitotic spindle assembly checkpoint]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007346 [regulation of mitotic cell cycle]
GO:0008017 [microtubule binding]
GO:0008283 [cell proliferation]
GO:0010800 [positive regulation of peptidyl-threonine phosphorylation]
GO:0010997 [anaphase-promoting complex binding]
GO:0015630 [microtubule cytoskeleton]
GO:0016301 [kinase activity]
GO:0016321 [female meiosis chromosome segregation]
GO:0016567 [protein ubiquitination]
GO:0018105 [peptidyl-serine phosphorylation]
GO:0019901 [protein kinase binding]
GO:0030071 [regulation of mitotic metaphase/anaphase transition]
GO:0030496 [midbody]
GO:0031145 [anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process]
GO:0031572 [G2 DNA damage checkpoint]
GO:0031648 [protein destabilization]
GO:0032436 [positive regulation of proteasomal ubiquitin-dependent protein catabolic process]
GO:0043066 [negative regulation of apoptotic process]
GO:0043393 [regulation of protein binding]
GO:0045143 [homologous chromosome segregation]
GO:0045184 [establishment of protein localization]
GO:0045736 [negative regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0045862 [positive regulation of proteolysis]
GO:0051233 [spindle midzone]
GO:0051297 [centrosome organization]
GO:0051437 [positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition]
GO:0051439 [regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0051443 [positive regulation of ubiquitin-protein transferase activity]
GO:0051726 [regulation of cell cycle]
GO:0070194 [synaptonemal complex disassembly]
GO:0071168 [protein localization to chromatin]
GO:1901673 [regulation of mitotic spindle assembly]
Show all
603 aa
68.3 kDa
No 0
PLK1-007
ENSP00000459688
ENST00000567897
I3L2H5 [Direct mapping]
Serine/threonine-protein kinase PLK1
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
Show all
105 aa
11.8 kDa
No 0
PLK1-008
ENSP00000460084
ENST00000568568
I3L309 [Direct mapping]
Serine/threonine-protein kinase PLK1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
Show all
58 aa
6.6 kDa
No 0

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