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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
Antibody dilution
1:62
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001861
Standard validation
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and nuclear positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001861
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
94.1, 81.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA001861
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA001861
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
VYPPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHP
QGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIF
PPLLPPTEQDLTKLLLEGQGESGGGSLGAQPLLQPSHYGQSGISMSHMDL
R
