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MTMR10

ANTIBODY INFORMATION
 
 

Antibody HPA006081

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006081  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA006081, RRID:AB_2667508  

VALIDATION SUMMARY
 
 

Antibody HPA006081

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006081

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.  

Antibody dilution

 1:38  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006081

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006081

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 88.3, 51.6  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA006081

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000  

ANTIGEN INFORMATION
 
 

Antibody HPA006081

  

Antigen

 Recombinant protein fragment  

Length (aa)

 118  

Antigen sequence

 
ESGPESAKKVCLAIAHYSQPTDLQLLFAFEYVGKKYHNSANKINGIPSGD
GGGGGGGGNGAGGGSSQKTPLFETYSDWDREIKRTGASGWRVCSINEGYM
ISTCLPEYIVVPSSLADQ
 

Matching transcripts

 MTMR10-001 - ENSP00000402537 [100%]
MTMR10-003 - ENSP00000457390 [100%]
 

Other gene match

  
ANTIGEN VIEW
MTMR10-001
MTMR10-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.