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ANTIBODY INFORMATION
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA004125   2287  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 3   4  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA004125, RRID:AB_1079312    

VALIDATION SUMMARY
 
 

Antibody HPA004125

  

Antibody CAB017097

  
Immunocytochemistry


Validated
   Supported  
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

 1:35    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

 1:35   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

 Approved    

   

Figure description

 Cytoplasmic positivity in subsets of neurons and their processes in several areas throughout the brain.    

WESTERN BLOT
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 101.1    

Loading control

 Total protein image    

Antibody dilution

 1:78    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 101.1   101.1, 26.6, 24.3, 12.6, 10.6, 7.6, 6.4, 6.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA004125

  

Antibody CAB017097

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 143    

Antigen sequence

 
FVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINA
VELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPN
AQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFD
  

											 
																				

																		

										
											
Matching transcripts

												
																					MARS-003 - ENSP00000262027 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MARS-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004125

												
										 
																				
											
											
											
Antibody CAB017097

												
										 
																		

																	

										
											
1.

												
																						Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype

	van Meel E et al

	BMC Med Genet 2013;14:106

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.