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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferous ducts and Leydig cells.
Expression
RNA: detected in 1 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Standard validation
Supported
Figure description
processes and cell bodies in specific regions, synaptic staining.
WESTERN BLOT
Antibody HPA043319
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407069)
Target mass (kDa)
39.2, 35
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA043319
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
