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ANTIBODY INFORMATION
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040593   HPA040902   HPA069773  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 8   8   16  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA040593, RRID:AB_10795525   Atlas Antibodies Cat#HPA040902, RRID:AB_10796230   Atlas Antibodies Cat#HPA069773, RRID:AB_2686194  

VALIDATION SUMMARY
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  
Immunocytochemistry

    Approved  
Immunohistochemistry


Validated
  

Validated
    
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.  

Antibody dilution

     1:114  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA040902 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA040593 across 75 cell types.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 51 cell types		   RNA: detected in 34 tissues
Protein: detected in 56 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:400   1:400    

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 213.7, 112   213.7, 112   213.7, 112, 82.4, 48  

Antibody dilution

 1:250   1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000   1:4550  

ANTIGEN INFORMATION
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   95   26  

Antigen sequence

 
VTTSTYTMEVERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSER
KILEDAHSREKDELRKRVADLEQENALLKDEKEQLNNQILCQSK
  
NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSN
QSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
  
PFNPSSLTMDSIHIPACLNLEFLNEV
 

Matching transcripts

 MYO5B-001 - ENSP00000285039 [100%]
MYO5B-006 - ENSP00000315531 [100%]
  MYO5B-001 - ENSP00000285039 [100%]
MYO5B-006 - ENSP00000315531 [100%]
  MYO5B-001 - ENSP00000285039 [100%]
MYO5B-002 - ENSP00000466368 [100%]
MYO5B-006 - ENSP00000315531 [100%]
MYO5B-201 - ENSP00000479038 [100%]
 

Other gene match

      
ANTIGEN VIEW
MYO5B-001
MYO5B-002
MYO5B-006
MYO5B-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA040593

  

Antibody HPA040902

  

Antibody HPA069773

  

1.

   Myo5b knockout mice as a model of microvillus inclusion disease
Cartón-García F et al
Sci Rep 2015;5:12312
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.