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PLIN4

ANTIBODY INFORMATION
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044682   HPA054657  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA044682, RRID:AB_2679044   Atlas Antibodies Cat#HPA054657, RRID:AB_2682559  

VALIDATION SUMMARY
 
 

Antibody HPA044682

  

Antibody HPA054657

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA054657.		  
Validated

Antibody staining overlaps with antibody HPA044682.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane, cytosol & lipid droplets.   Immunofluorescent staining of human cell line RT4 shows localization to cytosol & lipid droplets.  

Antibody dilution

 1:81   1:52  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA054657 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA044682 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA054657 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA044682 across 73 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in adipocytes.   Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in adipocytes.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 4 cell types		   RNA: detected in 31 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:4000   1:6000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 136, 134.4   136, 134.4  

Antibody dilution

 1:250   1:260  

PROTEIN ARRAY
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2050  

ANTIGEN INFORMATION
 
 

Antibody HPA044682

  

Antibody HPA054657

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 111   82  

Antigen sequence

 
GVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVC
SGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTG
LNTTQNIATGT
  
KGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTG
TKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLT
 

Matching transcripts

 PLIN4-001 - ENSP00000488481 [100%]
PLIN4-201 - ENSP00000301286 [100%]
  PLIN4-001 - ENSP00000488481 [100%]
PLIN4-201 - ENSP00000301286 [100%]
 

Other gene match

    
ANTIGEN VIEW
PLIN4-001
PLIN4-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA044682

  

Antibody HPA054657

  

1.

 Perilipin 4 in human skeletal muscle: localization and effect of physical activity
Pourteymour S et al
Physiol Rep 2015;3(8):
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.