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Immunohistochemical staining of human placenta shows strong membranous and cytoplamsic positivity in trophoblastic cells.
Expression
RNA: detected in 33 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021564
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407596)
Target mass (kDa)
63.1, 62.7, 48
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA021564
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
