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SERPINF2

ANTIBODY INFORMATION
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA001885   MAB1470  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   6  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA001885, RRID:AB_1079922    

VALIDATION SUMMARY
 
 

Antibody HPA001885

  

Antibody CAB024863

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.    

Antibody dilution

 1:7    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human esophagus shows distinct positivity in plasma.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 61 cell types		   RNA: detected in 21 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 54.6, 47.9, 29.5   54.6, 47.9, 29.5, 27.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA001885

  

Antibody CAB024863

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 122    

Antigen sequence

 
VPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQ
VLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQA
PDLRGISEQSLVVSGVQHQSTL
  

											 
																				

																		

										
											
Matching transcripts

												
																					SERPINF2-001 - ENSP00000371493 [100%] 
SERPINF2-004 - ENSP00000403877 [100%] 
SERPINF2-201 - ENSP00000321853 [100%] 
SERPINF2-003 - ENSP00000402056 [97%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SERPINF2-001
SERPINF2-003
SERPINF2-004
SERPINF2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001885

												
										 
																				
											
											
											
Antibody CAB024863

												
										 
																		

																	

										
											
1.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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