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HSD11B1L

ANTIBODY INFORMATION
 
 

Antibody HPA049078

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049078  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 16  

References

  

Proper citation

 Atlas Antibodies Cat#HPA049078, RRID:AB_2680622  

VALIDATION SUMMARY
 
 

Antibody HPA049078

  
Immunocytochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049078

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.  

Antibody dilution

 1:5  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA049078

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35.9, 34.3, 30.8, 25.8, 22.3, 21.5, 14.5  

Antibody dilution

 1:80  

PROTEIN ARRAY
 
 

Antibody HPA049078

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA049078

  

Antigen

 Recombinant protein fragment  

Length (aa)

 71  

Antigen sequence

 
DYLVLNHIGGAPAGTRARSPQATRWLMQVNFVSYVQLTSRALPSLTDSKG
SLVVVSSLLGRVPTSFSTPYS
 

Matching transcripts

 HSD11B1L-001 - ENSP00000340436 [100%]
HSD11B1L-002 - ENSP00000407154 [100%]
HSD11B1L-003 - ENSP00000301382 [100%]
HSD11B1L-004 - ENSP00000463073 [100%]
HSD11B1L-006 - ENSP00000462975 [100%]
HSD11B1L-007 - ENSP00000463794 [100%]
HSD11B1L-012 - ENSP00000343451 [100%]
HSD11B1L-021 - ENSP00000462921 [100%]
HSD11B1L-201 - ENSP00000480443 [100%]
 

Other gene match

  
ANTIGEN VIEW
HSD11B1L-001
HSD11B1L-002
HSD11B1L-003
HSD11B1L-004
HSD11B1L-006
HSD11B1L-007
HSD11B1L-012
HSD11B1L-021
HSD11B1L-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.