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SRRM2

ANTIBODY INFORMATION
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041411   HPA066181  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041411, RRID:AB_10796671   Atlas Antibodies Cat#HPA066181, RRID:AB_2685630  

VALIDATION SUMMARY
 
 

Antibody HPA041411

  

Antibody HPA066181

  
Immunocytochemistry


Validated

Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:73    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA066181.		  
Validated

Antibody staining overlaps with antibody HPA041411.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.   Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles.  

Antibody dilution

 1:73   1:31  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:600    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 299.6   299.6, 113.7, 99.7, 34.2, 32.6, 27.9, 10.5  

Antibody dilution

 1:250   1:100  

PROTEIN ARRAY
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1100  

ANTIGEN INFORMATION
 
 

Antibody HPA041411

  

Antibody HPA066181

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 106   72  

Antigen sequence

 
RSRSSSPVTELASRSPIRQDRGEFSASPMLKSGMSPEQSRFQSDSSSYPT
VDSNSLLGQSRLETAESKEKMALPPQEDATASPPRQKDKFSPFPVQDRPE
SSLVFK
  
LMLLEKDVNPGGKEETPGQRPAVTETHQLAELNEKKNERLRAAFGISDSY
VDGSSFDPQRRAREAKQPAPEP
 

Matching transcripts

 SRRM2-001 - ENSP00000301740 [100%]
  SRRM2-001 - ENSP00000301740 [100%]
SRRM2-003 - ENSP00000461181 [100%]
SRRM2-006 - ENSP00000458870 [99%]
SRRM2-007 - ENSP00000458647 [99%]
SRRM2-013 - ENSP00000484503 [99%]
SRRM2-028 - ENSP00000458718 [99%]
SRRM2-201 - ENSP00000487247 [99%]
 

Other gene match

    
ANTIGEN VIEW
SRRM2-001
SRRM2-003
SRRM2-006
SRRM2-007
SRRM2-013
SRRM2-028
SRRM2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.