SETD5 - Antibodies - The Human Protein Atlas

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SETD5

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035574

Antibody CAB017093

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA035574

2256

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035574, RRID:AB_2674681

VALIDATION SUMMARY

Antibody HPA035574

Antibody CAB017093

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035574

Antibody CAB017093

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:27

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.

Antibody dilution

1:27

1:13

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA035574

Antibody CAB017093

Standard validation

Approved

Figure description

Immunohistochemical staining of human nasopharynx shows strong nuclear positivity in respiratory epithelial cells.

Immunohistochemical staining of human nasopharynx shows nuclear positivity in respiratory epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:50

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035574

Antibody CAB017093

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

159.4, 157.5, 147.4, 99.7

Loading control

Total protein image

Antibody dilution

1:59

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

159.4, 157.5, 147.4, 99.7

159.4, 157.5, 147.4, 99.7, 48.1, 19.3, 17.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA035574

Antibody CAB017093

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA035574

Antibody CAB017093

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

ETPVGEETKTEAPESEVSNSVSNVTIPSTPQSVGVNTRRSSQAGDIAAEK
LVPKPPPAKPSRPRPKSRISRYRTSSAQRLKRQKQ

Matching transcripts

SETD5-001 - ENSP00000383939 [100%]
SETD5-003 - ENSP00000302028 [100%]
SETD5-004 - ENSP00000384114 [100%]
SETD5-015 - ENSP00000382593 [100%]
SETD5-201 - ENSP00000385852 [100%]

Other gene match

ANTIGEN VIEW

SETD5-001	SETD5-003	SETD5-004	SETD5-015	SETD5-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.