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ANTIBODY INFORMATION
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA036744   HPA036745   sc-65243  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 7   13   13  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA036744, RRID:AB_10670921   Atlas Antibodies Cat#HPA036745, RRID:AB_2675285   Santa Cruz Biotechnology Cat#sc-65243, RRID:AB_2162946  

VALIDATION SUMMARY
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  
Immunocytochemistry

  Supported    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.    

Antibody dilution

   1:91    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA036745 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA036744 across 46 cell lines.		    
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA036745 across 69 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA036744 across 69 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:600   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.2, 38.4, 37.2   39.2, 38.4, 37.5, 37.2   39.2, 38.4, 37.5, 37.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036744

  

Antibody HPA036745

  

Antibody CAB033794

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 85   77    

Antigen sequence

 
MAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGA
SAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKG
  
LIKSAIRDNNPVVVLENELMYGVPFEFPPEAQSKDFLIPIGKAKIERQGT
HITVVSHSRPVGHCLEAAAVLSKEGVE
  

											 
																				

																		

										
											
Matching transcripts

												
																					PDHB-001 - ENSP00000307241 [100%] 
PDHB-007 - ENSP00000418448 [100%] 
PDHB-008 - ENSP00000373220 [100%] 

											 
																						PDHB-001 - ENSP00000307241 [100%] 
PDHB-005 - ENSP00000417267 [100%] 
PDHB-007 - ENSP00000418448 [100%] 
PDHB-008 - ENSP00000373220 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PDHB-001
PDHB-005
PDHB-007
PDHB-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA036744

												
										 
																				
											
											
											
Antibody HPA036745

												
										 
																				
											
											
											
Antibody CAB033794

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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