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Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Antibody dilution
1:79
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038280
Antibody HPA064845
Standard validation
Approved
Figure description
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 35 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038280
Antibody HPA064845
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418651)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418651)
Target mass (kDa)
76.8, 75.1, 19.8
76.8, 75.1
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA038280
Antibody HPA064845
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
