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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:33
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA046245
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human esophagus shows strong nuclear, cytoplasmic and membranous positivity in superficial layer of squamous epithelial cells.
Expression
RNA: detected in 26 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046245
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
209.5, 177.9, 173.3, 108.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA046245
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
