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ANTIBODY INFORMATION
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Epitomics
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001671   HPA058603   1122-1   AMAb90777   AMAb90776  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 2   13   2   13   13  

References

 2          

Proper citation

 Atlas Antibodies Cat#HPA001671, RRID:AB_1080102   Atlas Antibodies Cat#HPA058603, RRID:AB_2683772     Atlas Antibodies Cat#AMAb90777, RRID:AB_2665663   Atlas Antibodies Cat#AMAb90776, RRID:AB_2665662  

VALIDATION SUMMARY
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  
Immunocytochemistry

Supported          
Immunohistochemistry


Validated

Validated
  

Validated
  

Validated
   Supported  

Validated
  
Western blot

Supported   Supported   Supported   Supported   Supported  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Standard validation

 Supported          

         

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.          

Antibody dilution

 1:15          

Literature conformity

 The subcellular location is supported by literature.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
   
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		        

 
       

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA058603 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA001671 across 46 cell lines.		        
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		        

 
       

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA058603 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA001671 across 75 cell types.		        
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human placenta shows strong nuclear positivity and cytoplasmic in decidual cells.   Immunohistochemical staining of human small intestine shows strong positivity in glandular cells.   Immunohistochemical staining of human lung shows distinct nuclear positivity in pneumocytes.   Immunohistochemical staining of human liver shows strong positivity in hepatocytes.   Immunohistochemical staining of human small intestine shows moderate cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:100   1:75   1:1200   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

         

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 88.1, 88, 76.1   88.1, 88, 83.1, 76.1   88.1, 88, 83.1, 76.1, 10.2   88.1, 88, 83.1, 76.1, 10.2   88.1, 88, 83.1, 76.1, 10.2  

Antibody dilution

 1:250   1:110   1:500   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:1000   1:1200        

ANTIGEN INFORMATION
 
 

Antibody HPA001671

  

Antibody HPA058603

  

Antibody CAB003859

  

Antibody CAB068241

  

Antibody CAB068242

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Recombinant protein   Recombinant protein  

Length (aa)

 149   138        

Antigen sequence

 
GVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILV
SPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTT
CSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECA
  
EKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLE
QHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSV
TRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQK
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					STAT3-001 - ENSP00000264657 [100%] 
STAT3-011 - ENSP00000467985 [100%] 
STAT3-013 - ENSP00000373923 [100%] 
STAT3-002 - ENSP00000384943 [99%] 

											 
																						STAT3-001 - ENSP00000264657 [100%] 
STAT3-002 - ENSP00000384943 [100%] 
STAT3-011 - ENSP00000467985 [100%] 
STAT3-012 - ENSP00000467000 [100%] 
STAT3-013 - ENSP00000373923 [98%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
STAT3-001
STAT3-002
STAT3-011
STAT3-012
STAT3-013
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001671

												
										 
																				
											
											
											
Antibody HPA058603

												
										 
																				
											
											
											
Antibody CAB003859

												
										 
																				
											
											
											
Antibody CAB068241

												
										 
																				
											
											
											
Antibody CAB068242

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Differentially expressed proteins in malignant and benign adrenocortical tumors

	Kjellin H et al

	PLoS One 2014;9(2):e87951

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
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