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ANTIBODY INFORMATION
 
 

Antibody CAB012283

  

Antibody CAB017783

  

Provider

 Abcam plc
   Origene
  

Product name

 ab32197   2483.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 msAb   msAb  

Purity

 Affinity   Affinity  

Other gene match

    

Released in version

 4   4  

References

    

Proper citation

 Abcam Cat#ab32197, RRID:AB_2290204    

VALIDATION SUMMARY
 
 

Antibody CAB012283

  

Antibody CAB017783

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB012283

  

Antibody CAB017783

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.  

Antibody dilution

   1:19  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB012283

  

Antibody CAB017783

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows nuclear and cytoplasmic positivity in neuronal cells and glial cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic and nuclear positivity.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 73 cell types		   RNA: detected in 35 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:175   1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB012283

  

Antibody CAB017783

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 93.6, 86.7, 30.1, 21.6, 18, 16.8   93.6, 86.7, 30.1, 21.6, 18, 16.8  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB012283

  

Antibody CAB017783

  

Antigen

 Synthetic peptide   Genetic immunization  

Length (aa)

    

Antigen sequence

 

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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