Fields »
ABOUT
HELP
BLOG
TM4SF20

ANTIBODY INFORMATION
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051838   HPA063184   HPA065531  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 14   14   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA051838, RRID:AB_2681631   Atlas Antibodies Cat#HPA063184, RRID:AB_2684958   Atlas Antibodies Cat#HPA065531, RRID:AB_2685506  

VALIDATION SUMMARY
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane & focal adhesion sites.  

Antibody dilution

     1:48  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous glandular cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.    

Expression

 RNA: detected in 6 tissues
Protein: detected in 35 cell types		   RNA: detected in 6 tissues
Protein: detected in 64 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:110   1:75    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 25.1   25.1   25.1  

Antibody dilution

 1:250   1:80   1:160  

PROTEIN ARRAY
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2900   1:500   1:1900  

ANTIGEN INFORMATION
 
 

Antibody HPA051838

  

Antibody HPA063184

  

Antibody HPA065531

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 56   30   56  

Antigen sequence

 
IHPESFNLQWFFNDSCAPPTGFNKPTSNDTMASGWRASSFHFDSEENKHR
LIHFSV
  
QALLKGPLMCNSPSNSNANCEFSLKNISDI
  
IHPESFNLQWFFNDSCAPPTGFNKPTSNDTMASGWRASSFHFDSEENKHR
LIHFSV
 

Matching transcripts

 TM4SF20-001 - ENSP00000303028 [100%]
  TM4SF20-001 - ENSP00000303028 [100%]
  TM4SF20-001 - ENSP00000303028 [100%]
 

Other gene match

      
ANTIGEN VIEW
TM4SF20-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.