Fields »
ABOUT
HELP
BLOG
CD2BP2

ANTIBODY INFORMATION
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041508   HPA061309  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041508, RRID:AB_2677523   Atlas Antibodies Cat#HPA061309, RRID:AB_2684476  

VALIDATION SUMMARY
 
 

Antibody HPA041508

  

Antibody HPA061309

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & nucleoli fibrillar center.  

Antibody dilution

 1:200   1:41  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA061309 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA041508 across 73 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows nuclear positivity in germinal center.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 52 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:250  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 37.6   37.6  

Antibody dilution

 1:250   1:140  

PROTEIN ARRAY
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA041508

  

Antibody HPA061309

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 104   52  

Antigen sequence

 
LGPHNPTPPPSLDMFAEELAEEELETPTPTQRGEAESRGDGLVDVMWEYK
WENTGDAELYGPFTSAQMQTWVSEGYFPDGVYCRKLDPPGGQFYNSKRID
FDLY
  
MPKRKVTFQGVGDEEDEDEIIVPKKKLVDPVAGSGGPGSRFKGKHSLDSD
EE
 

Matching transcripts

 CD2BP2-001 - ENSP00000304903 [100%]
CD2BP2-002 - ENSP00000456935 [100%]
  CD2BP2-001 - ENSP00000304903 [100%]
CD2BP2-002 - ENSP00000456935 [100%]
 

Other gene match

    
ANTIGEN VIEW
CD2BP2-001
CD2BP2-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.