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Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
Antibody dilution
1:87
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody CAB005004
Standard validation
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in pancreatic ducts and nuclear membranous positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
Antibody dilution
1:350
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody CAB005004
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
